DNA

Part:BBa_K1039028:Design

Designed by: Jama Hagi-Yusuf   Group: iGEM13_Waterloo   (2013-09-13)


PhiC31 Invertible Medium Strength Promoter Switch Test Construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 751
    Illegal NheI site found at 937
    Illegal NheI site found at 960
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1844
    Illegal AgeI site found at 1956
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 74


Design Notes

Note that there is an NheI site on the prefix side of the switch before the attP site. This was placed there as part of an early design of our switch, but does not contribute to functionality.


Source

The parts were either synthesized by BioBasic Inc or from the parts registry.

References